This article shows how enzymatic activity can be effectively determined by means of the Eppendorf BioSpectrometer. The preliminary measurements were carried out by utilizing the “single λ-continuous” ...

Temperature and substrate availability constrain the activity of the extracellular enzymes that decompose and release nutrients from soil organic matter (SOM). Proteolytic enzymes are the primary ...

Enzymes evolved to efficiently operate in low-temperature environments via key substitutions of amino acids in their active site, which lowers the activation energy of catalytic reactions. Life has ...

Scientists studying a COVID-19 coronavirus enzyme at temperatures ranging from frosty to human-body warm discovered subtle structural shifts that offer clues about how the enzyme works. The findings ...

Note: this practical uses the temperature of 35°C. This is close to body temperature. Warmer temperatures may denature the enzymes and the reaction will take longer at lower temperatures.

A pharmaceutical scientist at the National University of Singapore (NUS) has developed a method that can measure the kinetic efficiency of an enzyme against more than 200,000 potential peptide ...

This accurate thermosensation relies on the precise maintenance of thermal receptor functions; however, their activation and regulatory mechanisms have remained unclear. In a new study, researchers ...