Absorbance methods measure the amount of light absorbed by a sample at a specific wavelength. The absorbance is directly proportional to the sample's concentration, enabling accurate quantification.

A routine analysis performed in life science laboratories is the measurement of protein concentration in liquid samples. Accurate measurement is often essential for subsequent analyses such as western ...

Fluorescence spectra are often subject to significant distortions. These are largely predictable and are caused by concentration-dependent Inner Filter Effects (IFE). It is therefore the ...